Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
4 years ago

Spata-13,17-diene Synthase, an Enzyme with Sesqui-, Di- and Sesterterpene Synthase Activity from Streptomyces xinghaiensis

Lukas Lauterbach, Jan Rinkel, Jeroen Sidney Dickschat
A terpene synthase from the marine bacterium Streptomyces xinghaiensis was characterised, including a full structure elucidation of its products from various substrates and an in-depth investigation of the enzyme mechanism by isotopic labelling experiments, substrate and metal cofactor variations, and mutation experiments. The results revealed an interesting dependency of Mn2+ catalysis on the presence of Asp-217, a residue that is occupied by a highly conserved Glu in most other bacterial terpene synthases.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201711142

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