Lysosome-Targeting Amplifiers of Reactive Oxygen Species as Anticancer Prodrugs

Angewandte Chemie - International Edition

5 years ago

Lysosome-Targeting Amplifiers of Reactive Oxygen Species as Anticancer Prodrugs

Christoph Alexiou, Steffen Daum, Leopold Sellner, Tetyana Dumych, Maxim D. Lootsik, Christina Janko, Andriy Mokhir, Martin Herrmann, Evgenia Bila, Miroslav Sisa, Rostyslav Bilyy, M. S. Viktor Reshetnikov

Cancer cells produce elevated levels of reactive oxygen species, which has been used to design cancer specific prodrugs. Their activation relies on at least a bimolecular process, in which a prodrug reacts with ROS. However, at low micromolar concentrations of the prodrugs and ROS, the activation is usually inefficient. Herein, we propose and validate a potentially general approach for solving this intrinsic problem of ROS-dependent prodrugs. In particular, known prodrug 4-(N-ferrocenyl-N-benzylaminocarbonyloxymethyl)phenylboronic acid pinacol ester was converted into its lysosome-specific analogue. Since lysosomes contain a higher concentration of active ROS than the cytoplasm, activation of the prodrug was facilitated with respect to the parent compound. Moreover, it was found to exhibit high anticancer activity in a variety of cancer cell lines (IC50=3.5–7.2 μm) and in vivo (40 mg kg−1, NK/Ly murine model) but remained weakly toxic towards non-malignant cells (IC50=15–30 μm). Targeting cancer: A potentially general approach for improving the anti-cancer activity of ROS-dependent prodrugs is presented and validated. In particular, a known prodrug was converted into its lysosome-specific analogue. Because lysosomes contain a higher concentration of active ROS than the cytoplasm, activation of the prodrug was facilitated with respect to the parent compound.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201706585