4 years ago

Coupling Molecular Photocatalysis to Enzymatic Conversion

Coupling Molecular Photocatalysis to Enzymatic Conversion
Sven Rau, Bernhard J. Eikmanns, Alexander K. Mengele, Gerd M. Seibold
A hetero-binuclear dyad that contains a ruthenium polypyridyl moiety bound through an aromatic bridging ligand to an organometallic catalytic center has been used for the light-driven reduction of the N-benzyl-3-carbamoylpyridinium cation, NAD+, and NADP+ to yield the two-electron-reduced analog. Direct coupling with enzymatic conversion was proved by using UV/Vis spectroscopy and liquid chromatography, which showed cofactor-recycling and enzymatic conversion with a turnover number of 350 per photocatalyst. First insights into the complex behavior of the catalytic system under irradiation point towards multiple prerequisites on the molecular as well as on the macroscopic level to generate highly efficient semiartificial photo-biocatalytic systems for future energy-storage applications. Cofactor recycling: A hetero-binuclear photocatalyst drives the enzymatic reduction of pyruvate to lactate by the continuous generation of NADH under visible-light irradiation.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cctc.201701232

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.