Chemical Biology and Drug Design
Chemical Biology and Drug Design
5 years ago

The Effect of Chrysin Loaded Nanofiber on Wound Healing Process in Male Rat

The Effect of Chrysin Loaded Nanofiber on Wound Healing Process in Male Rat
Zoheyr Mohammadi, Hasan Majdi, Abolfazl Akbarzadeh, Khaled Seidi, Mohsen Sharif Zak, Ali Mohammad Latifi, Meisam Barati
Aim Wound healing is an inflammatory process. Chrysin, a natural flavonoid found in honey, has been recently investigated to have anti-inflammatory and antioxidant effects. In the current work, the effects of Chrysin loaded nanofiber on the expressions of genes that are related to wound healing process such as P53, TIMPs, MMPs, iNOS and IL-6 in an animal model study were evaluated. Material and methods The electrospinning method was used for preparation the different concentrations of Chrysin-loaded PCL-PEG nanofiber (5%, 10%, and 20% (w/w)) and characterized by FTIR and SEM. The wound healing effect of Chrysin-loaded PCL-PEG nanofiber were in vivo investigated in rats, and the expressions of genes related to wound healing process were evaluated by real-time PCR Results The study results showed Chrysin-loaded PLC-PEG compared to Chrysin ointment and control groups significantly increase IL-6, MMP-2, MMP-8, MMP-9, TIMP-1 and TIMP-2 (P<0.05). On the other hand nanofibers containing Chrysin significantly decreased p53 and iNOS expression compared to Chrysin ointment and control groups (P<0.05). Conclusion According to the results, Chrysin-loaded PCL-PEG-PCL nanofiber have positive effects on the expression of the genes that have pivotal role in wound healing. This article is protected by copyright. All rights reserved. The average diameter of PCL-PEG nanofibers and Chrysin loaded PCL-PEG nanofibers were about the range of 50-150nm. It was observed from these micrographs that the nanoparticles of PCL-PEG exhibited smooth surfaces and uniform structures with beads Morphology of PCL-PEG nanofibres: (A) free of Chrysin; (B) 5% (w/w) Chrysin-loaded PCL-PEG nanofiber; (C) 10% (w/w) Chrysin-loaded PCL-PEG nanofiber; (D) 15% (w/w) Chrysin-loaded PCL-PEG nanofiber

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/cbdd.12996

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