Chemical Biology and Drug Design
Chemical Biology and Drug Design
5 years ago

OM-LV20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo

OM-LV20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo
Yunshan Su, Ying Wang, Meifeng Yang, Jing Tang, Xiaojie Li, Zhirong Zou, Xinwang Yang, Chunyun Wu
The healing of chronic wounds remains a considerable challenge in clinical trials and imposes severe financial and physiological burdens on patients. Many works are being tried to find ideal clinical promoting wound healing biomaterials. Small bioactive peptides with low cost and easy production, store and transfer become excellent candidates. Here, we identified a novel peptide (named OM-LV20) from skin secretions of odorous frog Odorrana margaretae. The peptide had an amino acid sequence of “LVGKLLKGAVGDVCGLLPIC,” contained an intramolecular disulfide bridge at the C-terminus, and was produced by post-translational processing of a 71-residue prepropeptide. Our results showed that OM-LV20 had no direct microbe-killing effects, hemolytic activity, or acute toxicity, but did exhibit weak antioxidant activity. OM-LV20 promoted wound healing against human keratinocytes (HaCaT) and human skin fibroblasts (HSF) in both time- and dose-dependent manners. In addition, it induced the proliferation of HaCaT but not HSF cells. Of note, OM-LV20 showed strong wound healing-promoting activity in a mice model of full-thickness skin wound. Our research indicates the cellular and animal level wound healing potential of OM-LV20, and thus provides a novel bioactive peptide template for the development of wound healing agents and medicine. Odorous frog skin-derived OM-LV20 showed wound healing activities on cellular and animal levels. OM-LV20 showed no antimicrobial, hemolytic, and acute but weak antioxidant activities. The sequence of OM-LV20 was “LVGKLLKGAVGDVCGLLPIC,” and it contained an intermolecular cyclic motif located at C-terminus.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/cbdd.13063

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