5 years ago

Carboxymethylated Dextran-Modified N-Heterocyclic Carbene Self-Assembled Monolayers on Gold for Use in Surface Plasmon Resonance Biosensing

Carboxymethylated Dextran-Modified N-Heterocyclic Carbene Self-Assembled Monolayers on Gold for Use in Surface Plasmon Resonance Biosensing
Hongxia Hao, Kim Munro, Bin Hao, J. Hugh Horton, Cathleen M. Crudden, Zhijun Li, Mina R. Narouz
Surface chemistry is a key enabler for various biosensing applications. Biosensors based on surface plasmon resonance routinely employ thiol-based chemistry for the linker layer between gold-coated support surfaces and functional biosensor surfaces. However, there is a growing awareness that such sensor surfaces are prone to oxidation/degradation problems in the presence of oxygen, and previous efforts to improve the stability have shown limited advancements. As an alternative, recent studies employing N-heterocyclic carbene (NHC) self-assembled monolayers (SAMs) deposited on gold have shown significant promise in this area. Here, we describe a sensor surface employing an NHC SAM to couple a modified carboxymethylated dextran onto a gold surface. Such a dextran matrix is also used for affinity chromatography, and it is the most commonly employed matrix for commercial biosensor surfaces today. The performance reliability of the dextran-modified NHC chip to act as an alternative biosensing platform is compared with that of a thiol-based commercial chip in the proof-of-concept tests. The resultant NHC sensor surface shows a higher thermal stability compared to thiol analogues. Moreover, the plasma protein/drug and antibody/antigen interactions were validated on the NHC-based dextran chip and showed similar performance as compared to the thiol-based commercial chip. Ultimately, this study shows the strong potential applicability of chemical modifications to gold surfaces using NHC ligands for biosensing applications.

Publisher URL: http://dx.doi.org/10.1021/acsami.7b13114

DOI: 10.1021/acsami.7b13114

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