5 years ago

Peptide-Mediated Membrane Transport of Macromolecular Cargo Driven by Membrane Asymmetry

Peptide-Mediated Membrane Transport of Macromolecular Cargo Driven by Membrane Asymmetry
Xin Li, Matthew A. Holden, Min Chen, Jing Huang
Pep-1 is a cell-penetrating peptide that represents a powerful strategy for delivering large, hydrophilic therapeutic molecules into cells. Model membranes, such as lipid vesicles and planar bilayers, have been useful for investigating the direct translocation of cell-penetrating peptides. Here, we present a droplet interface bilayer-based approach to quantify pep-1-mediated β-galactosidase translocation. We found that β-galactosidase translocation is driven only by the negative transmembrane potential resulting from the asymmetric bilayers. The asymmetric droplet interface bilayer method may be generally applicable for high-throughput screening of the efficacy of cell-penetrating peptides.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b03421

DOI: 10.1021/acs.analchem.7b03421

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