5 years ago

Use of Accelerate Pheno™ System for Identification and Antimicrobial Susceptibility Testing (ID/AST) of Pathogens in Positive Blood Cultures and Impact on Time to Results and Workflow.

Nedra Love, Angella Charnot-Katsikas, Kathleen G Beavis, Sue Boonlayangoor, Cindy Bethel, Vera Tesic, Brandy Hill
The Accelerate Pheno™ system uses automated fluorescence in situ hybridization technology with morphokinetic cellular analysis to provide rapid species identification (ID) and antimicrobial susceptibility testing (AST) results for the most commonly identified organisms in bloodstream infections.Objective was to evaluate the accuracy and workflow of bacterial and yeast ID, and bacterial AST using the Accelerate Pheno™ system in the clinical microbiology laboratory.Consecutive fresh blood cultures received in the laboratory were analyzed by the Accelerate Pheno™ system within 0 - 8 h of growth detection. ID/AST performance, the average times to results, and workflow were compared to the routine standard of care.Of the 232 blood cultures evaluated (223 monomicrobial, 9 polymicrobial) comprising 241 organisms, overall sensitivity and specificity for identification of organisms was 95.6% and 99.5%, respectively. For antimicrobial susceptibility, overall essential agreement was 95.1% and categorical agreement was 95.5%, compared to routine methods. There was one very major error and 3 major errors. Time to identification and time to susceptibility using the Accelerate Pheno™ system were decreased by 23.47 and 41.86 h, respectively, when compared to standard of care. Reduction in hands on time was 25.5 min per culture.The Accelerate Pheno™ system provides rapid and accurate ID/AST results for most of the organisms found routinely in blood cultures. It is easy to use, reduces hands on time for ID/AST of common blood pathogens, and allows clinically actionable results to be released much earlier than the current standard of care.

Publisher URL: http://doi.org/10.1128/JCM.01166-17

DOI: 10.1128/JCM.01166-17

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