4 years ago

In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis.

Sarah El-Messeiry, Emmanouela Stamatidou, Andrew Hamilton, Christopher Estell
Although mitochondrial translation produces only 13 proteins, we show here how this can be labelled and detected in situ by fluorescence microscopy with a simple, rapid and inexpensive procedure using non-canonical amino acid labelling and click chemistry. This allows visualisation of the translational output in different mitochondria within a cell, their position within that cell and a comparison of mitochondrial translation between cells. Most highly translationally active mitochondria were closest to the nucleus but were also found at the distal end of long cellular projections. There were substantial differences in translation between adjacent mitochondria and this did not readily correlate with apparent Mt-genome content. Mitochondrial translation was unchanged during mitosis when cytoplasmic translation was suppressed. This method will serve both fundamental cell biology and clinically orientated studies in which mitochondrial function is a key parameter.

Publisher URL: http://doi.org/10.1242/jcs.206714

DOI: 10.1242/jcs.206714

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