5 years ago

Two members of the velvet family, VmVeA and VmVelB, affect conidiation, virulence and pectinase expression in Valsa mali

Liangsheng Xu, Ralf T. Voegele, Qingqing Dai, Yuxing Wu, Lili Huang, Zhiyuan Yin, Xiaoning Gao, Hao Feng
Velvet protein family members are important fungal-specific regulators that are involved in conidial development, secondary metabolism, and virulence. To gain broader insight into the physiological functions into the velvet protein family of Valsa mali, which causes a highly destructive canker disease on apple, we conducted a functional analysis of two Velvet protein family members (VmVeA and VmVelB) via gene replacement strategy. Deletion mutants of VmVeA and VmVelB showed increased melanin production, conidiation, and sensitivity to abiotic stresses, but exhibited reduced virulence on detached apple leaves and twigs. Further studies demonstrated that the regulation of conidiation by VmVeA or VmVelB was positively correlated with melanin synthesis transcription factor VmCmr1. More importantly, transcript levels of pectinase genes were shown to be decreased in deletion mutants compared to those of the wild type during infection. However, the expression of other cell wall-degrading enzymes including cellulase, hemi-cellulase, or ligninase genes was not affected in the deletion mutants. Furthermore, the determination of pectinase activity and immunogold labeling of pectin demonstrated that the capacity of pectin degradation was attenuated due to deletions of VmVeA and VmVelB. Finally, the interaction of VmVeA with VmVelB was identified through co-immunoprecipitation assays. VmVeA and VmVelB play critical roles in conidiation and virulence likely by regulating melanin synthesis transcription factor VmCmr1 and affecting pectinase gene expression in V. mali, respectively. This article is protected by copyright. All rights reserved.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1111/mpp.12645

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.