Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
4 years ago

Fibroin/dodecanol floating solidification microextraction for the preconcentration of trace levels of flavonoids in complex matrix samples11Abbreviations: F/D-FSME, fibroin/dodecanol floating solidification microextraction; HPLC, high performance liquid chromatography; UV, ultraviolet detection; EF, enrichment factor; TCM, traditional Chinese medicine; LPE, liquid phase extraction; SPE, solid phase extraction; LPME, liquid phase microextraction; SPME, solid phase microextraction; SFODLPME, solidification of floating organic droplet liquid phase microextraction; D-FSME, dodecanol floating solidification microextraction.

A new fibroin/dodecanol floating solidification microextraction, coupled with high performance liquid chromatography, was developed and applied for enrichment and quantification of the trace flavonoids in traditional Chinese medicine and biological samples. Also, fibroin sensibilization mechanism was described, and influence of sample matrix to enrichment factor was investigated. In this method, a homogeneous fibroin/dodecanol of dispersed solution was employed as microextraction phase to flavonoids (myricetin, quercetin, isorhamnetin, chrysin, kaempferide), the several critical parameters affecting the performance, such as organic extractant, amount of fibroin in organic extractant, volume of extraction phase, dispersant, salt concentration, pH of sample phase, stirring rate, extraction time, and volume of sample phase were tested and optimized. Under the optimized conditions, enrichment factor of flavonoids ranged from 42.4 to 238.1 in different samples, excellent linearities with r2≥ 0.9968 for all analytes were achieved, limits of detection were less than or equal to 5.0ng/mL, average recoveries were 92.5% to 115.0% in different samples. The new procedure is simple, fast, low cost, environmentally friendly and high EF, it can also be applied to the concentration and enrichment of the trace flavonoids in other complex matrixes.

Publisher URL: www.sciencedirect.com/science

DOI: S1570023217305639

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