Stem Cell Research
Stem Cell Research
5 years ago

Generation of induced pluripotent stem cells from a patient with Parkinson's disease carrying LRRK2 p.I2012T mutation.

Yu-Hung Hsu, Ming-Ching Ho, Patrick C H Hsieh, Yu-Che Cheng, Huai-En Lu, Han-I Lin, Cheng-Hao Wen, Hui-Wen Ko, Ching-Ying Huang, Chin-Hsien Lin
Parkinson's disease (PD) is a progressive neurodegenerative disorder caused by interactions between genetic and environmental factors. Leucine rich repeat kinase (LRRK2) is the most prevalent mutation in autosomal-dominant inheritance of PD. Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with p.I2012T mutation in LRRK2 gene by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the 3 germ layers in vivo. This cellular model will provide a useful platform for further pathophysiological studies of PD.

Publisher URL: http://doi.org/10.1016/j.scr.2017.10.020

DOI: 10.1016/j.scr.2017.10.020

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