5 years ago

Oxidation of [U-13C]glucose in the human brain at 7T under steady state conditions

Ivan E. Dimitrov, A. Dean Sherry, Karthik Rajasekaran, Dorothy Kelly, Craig R. Malloy, Juan M. Pascual, Levi Good, Ralph J. DeBerardinis, Sergey Cheshkov, Vikram Jakkamsetti
Purpose Disorders of brain energy metabolism and neurotransmitter recycling have been implicated in multiple neurological conditions. 13C magnetic resonance spectroscopy (13C MRS) during intravenous administration of 13C-labeled compounds has been used to measure turnover rates of brain metabolites. This approach, however, requires prolonged infusion inside the magnet. Proton decoupling is typically required but may be difficult to implement with standard equipment. We examined an alternative approach to monitor glucose metabolism in the human brain. Methods 13C-enriched glucose was infused in healthy subjects outside the magnet to a steady-state level of 13C enrichment. Subsequently, the subjects were scanned at 7T for 60 min without 1H decoupling. Metabolic modeling was used to calculate anaplerosis. Results Biomarkers of energy metabolism and anaplerosis were detected. The glutamate C5 doublet provided information about glucose-derived acetyl-coenzyme A flux into the tricarboxylic acid (TCA) cycle via pyruvate dehydrogenase, and the bicarbonate signal reflected overall TCA cycle activity. The glutamate C1/C5 ratio is sensitive to anaplerosis. Conclusion Brain 13C MRS at 7T provides information about glucose oxidation and anaplerosis without the need of prolonged 13C infusions inside the scanner and without technical challenges of 1H decoupling, making it a feasible approach for clinical research. Magn Reson Med 78:2065–2071, 2017. © 2017 International Society for Magnetic Resonance in Medicine.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/mrm.26603

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.