5 years ago

Chemotaxis of V{delta}2 T cells to the joints contributes to the pathogenesis of rheumatoid arthritis


To explore the role of V2 T cells in the pathogenesis of rheumatoid arthritis (RA).


Sixty-eight patients with RA, 21 patients with osteoarthritis and 21 healthy controls were enrolled in the study. All patients with RA fulfilled the 2010 American College of Rheumatology/European League Against Rheumatism criteria for RA. Peripheral V2T population, chemokine receptor expression and proinflammatory cytokine secretion were quantified by flow cytometry. The infiltration of V2 T cells within the synovium was examined by immunohistochemistry and flow cytometry. The effect of tumour necrosis factor (TNF)-α and interleukin (IL)-6 on V2 T migration was determined by flow cytometry and transwell migration assay.


Peripheral V2T cells, but not V1 T cells, were significantly lower in patients with RA, which was negatively correlated with disease activity gauged by Disease Activity Score in 28 joints. V2 T cells from RA accumulated in the synovium and produced high levels of proinflammatory cytokines including interferon- and IL-17. Phenotypically, V2 T cells from RA showed elevated chemotaxis potential and expressed high levels of chemokine receptors CCR5 and CXCR3, which was driven by increased serum TNF-α through nuclear factor kappa B signalling. In vivo, TNF-α neutralising therapy dramatically downregulated CCR5 and CXCR3 on V2 T cells and repopulated the peripheral V2 T cells in patients with RA.


High levels of TNF-α promoted CCR5 and CXCR3 expression in V2 T cells from RA, which potentially infiltrated into the synovium and played crucial roles in the pathogenesis of RA. Targeting V2 T cells might be a potential approach for RA.

Publisher URL: http://ard.bmj.com/cgi/content/short/76/12/2075

DOI: 10.1136/annrheumdis-2016-211069

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.