5 years ago

Nanopatterned Adhesive, Stretchable Hydrogel to Control Ligand Spacing and Regulate Cell Spreading and Migration

Nanopatterned Adhesive, Stretchable Hydrogel to Control Ligand Spacing and Regulate Cell Spreading and Migration
Joachim P. Spatz, Jie Deng, Qiang Wei, Changsheng Zhao
Spatial molecular patterning enables the regulation of adhesion receptor clustering and can thus play a pivotal role in multiple biological activities such as cell adhesion, viability, proliferation, and differentiation. A wide range of nanopatterned, adhesive interfaces have been designed to decipher the essence of molecular-scale interactions between cells and the adhesive interface. Although an interligand spacing of less than 70 nm is a proven prerequisite for the formation of stable focal adhesions, there is a paucity of data concerning how cells behave on substrates featuring heterogeneous adhesiveness. In this study, a stretchable hydrogel functionalized with a quasi-hexagonally arranged nanoarray was stretched along one direction, resulting in ligands periodically arranged in a pattern resembling a centered rectangular lattice with an interligand spacing smaller than 70 nm in one direction and greater than 70 nm in the orthogonal direction. This substrate was utilized to modulate interligand spacing and investigate cell adhesion and migration. An interligand spacing larger than 70 nm—even in just one direction—prevented the establishment of stable focal adhesions. The stretched interface promoted dynamic remodeling at cell contacts, resulting in higher cellular mobility. Our nanopatterned stretchable hydrogel permits reversible control over cell adhesion and migration on nanopatterned ligand interfaces.

Publisher URL: http://dx.doi.org/10.1021/acsnano.7b03449

DOI: 10.1021/acsnano.7b03449

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.