Nature Chemical Biology
Nature Chemical Biology
4 years ago

Synthetic microbial consortia enable rapid assembly of pure translation machinery

Synthetic microbial consortia enable rapid assembly of pure translation machinery
Cheemeng Tan, Luis E Contreras-Llano, Tingrui Pan, Michael Chavez, Jinzhen Fan, Yunfeng Ding, Fernando Villarreal
Assembly of recombinant multiprotein systems requires multiple culturing and purification steps that scale linearly with the number of constituent proteins. This problem is particularly pronounced in the preparation of the 34 proteins involved in transcription and translation systems, which are fundamental biochemistry tools for reconstitution of cellular pathways ex vivo. Here, we engineer synthetic microbial consortia consisting of between 15 and 34 Escherichia coli strains to assemble the 34 proteins in a single culturing, lysis, and purification procedure. The expression of these proteins is controlled by synthetic genetic modules to produce the proteins at the correct ratios. We show that the pure multiprotein system is functional and reproducible, and has low protein contaminants. We also demonstrate its application in the screening of synthetic promoters and protease inhibitors. Our work establishes a novel strategy for producing pure translation machinery, which may be extended to the production of other multiprotein systems.

Publisher URL: https://www.nature.com/articles/nchembio.2514

DOI: 10.1038/nchembio.2514

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