Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
5 years ago

In Situ Scatheless Cell Detachment Reveals Connections Between Adhesion Strength and Viability at Single-Cell Resolution

Katsumi Uchiyama, Jin-Ming Lin, Qiushi Huang, Sifeng Mao, Masqooh Khan, Wanling Zhang, Haifang Li
Single-cell biology provides insights to some of the most fundamental processes in biology and promotes the understanding of life's mysteries. And as the technologies to study single-cells expand, they will require sophisticated analytical tools to make sense of various behaviors and components of single-cells as well as their relations in adherent tissue culture. In this paper, we revealed cell heterogeneity and uncovered the connections between cell adhesion strength and cell viability at single-cell resolution by extracting single adherent cells of interest from standard tissue culture via a microfluidic chip based Live Single-Cell Extractor (LSCE). We not only uncovered cell heterogeneity and the connections between cell adhesion strength and cell viability, but also provided a new methodology for single-cell biology.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201710273

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