Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
4 years ago

Photoluminescence Lifetime Imaging of Synthesized Proteins in Living Cells Using an Iridium–Alkyne Probe

Photoluminescence Lifetime Imaging of Synthesized Proteins in Living Cells Using an Iridium–Alkyne Probe
Jinyu Wang, Juan Qiao, Zihe Yan, Sichun Zhang, Jie Xue, Xinrong Zhang
Designing probes for real-time imaging of dynamic processes in living cells is a continuous challenge. Herein, a novel near-infrared (NIR) photoluminescence probe having a long lifetime was exploited for photoluminescence lifetime imaging (PLIM) using an iridium-alkyne complex. This probe offers the benefits of deep-red to NIR emission, a long Stokes shift, excellent cell penetration, low cytotoxicity, and good resistance to photobleaching. This example is the first PLIM probe applicable to the click reaction of copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC) with remarkable lifetime shifts of 414 ns, before and after click reaction. The approach fully eliminates the background interference and distinguishes the reacted probes from the unreacted probes, thus enabling the wash-free imaging of the newly synthesized proteins within single living cells. Based on the unique properties of the iridium complexes, it is anticipated to have applications for imaging other processes within living cells. It takes a lifetime: A novel bioorthogonal probe, an Ir-alkyne complex, was designed and synthesized. It showed deep-red to near-IR emission and unexpected large lifetime shifts of 414 ns before and after click reaction. This probe enabled the wash-free imaging of the newly synthesized proteins within living cells. NP=nanoparticle.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201708566

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