3 years ago

Effective Attenuation of Glyphosate Induced Oxidative Stress and Granulosa Cell Apoptosis by Vitamin C and E in Caprines

Jitender Kumar Bhardwaj, Meenu Mittal, Priyanka Saraf

Abstract

Pesticides are known to cause wide range of reproductive problems that possesses degenerative effects on mammalian fertility. Glyphosate (GLP), a broad‐spectrum organophosphate herbicide, is known to be a potent mammalian toxicant. The present study aims at assessing the GLP (0.1, 2.0 and 4.0 mg/ml) induced granulosa cells toxicity and evaluating the mitigating effects of Vitamins C & E (0.5mM and 1.0mM) in healthy caprine antral follicles, cultured in vitro in dose and time (24, 48 and 72 hr) dependent manner and subjected to various cytotoxic and geno‐toxic analysis namely classic histology, EB/AO differential staining, oxidative stress parameters and antioxidant enzymatic activity. Histomorphological analysis and EB/AO staining elucidated increase in incidence of apoptotic attributes within granulosa cells with increasing dose and duration of GLP treatment. Highest apoptotic frequency was observed at 4.0 mg/ml GLP post 72 hr exposure duration in comparison with control. GLP exposure also led to significant decline in the antioxidant enzymes’ activity namely SOD, Catalase and GST along with enhanced lipid peroxidation and reduced FRAP activity in dose and time dependent manner. Vitamin C & E supplementation decreased oxidative stress mediated granulosa cells apoptosis, suggesting its efficiency to diminish GLP mediated GCs cytotoxicity and thereby, preventing associated fertility disorders.

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