ChemBioChem
ChemBioChem
5 years ago

1,2,4-Triazine-Modified 2′-Deoxyuridine Triphosphate for Efficient Bioorthogonal Fluorescent Labeling of DNA

1,2,4-Triazine-Modified 2′-Deoxyuridine Triphosphate for Efficient Bioorthogonal Fluorescent Labeling of DNA
Hans-Achim Wagenknecht, Krisana Peewasan
The cover picture shows the gel image of the extension and biorthogonal labelling of a DNA primer by using polymerases, the newly synthesized triazine-modified uridine triphosphate, and the other three natural nucleoside triphosphates. Because of the fluorescein marker of the primer, the primer extension hat places the bioorthogonally reacting triazene groups into the elongated oligonucleotide, as can be seen by the significant gel shift of the green emitting bands. The subsequent biorthogonal labelling of the triazine groups by rhodamine induces an additional, smaller gel shift in the green-emitting bands but also adds red emission to these bands that can be observed by using a second fluorescence readout channel. More information can be found in the communication by H.-A. Wagenknecht and K. Peewasan (DOI: 10.1002/cbic.201700185).

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700370

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