ChemBioChem
ChemBioChem
5 years ago

Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor-Binding Element of Amine Transaminase

Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor-Binding Element of Amine Transaminase
Sebastian Bartsch, Patrick Adlercreutz, Tim Börner, Andreas Vogel, Sebastian Rämisch, Carl Grey
The back cover picture shows an engineered, highly stable amine transaminase that catalyses the enantioselective amination of ketones under process conditions, that is, high temperature and substrate concentration as well as solvent use. Through a semirational design, the two point mutations in the cofactor binding region (illustrated by the screws) prevent protein unfolding and cofactor loss (indicated by the chain) during catalysis. More information can be found in the communication by C. Grey et al. (DOI: 10.1002/cbic.201700236).

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700373

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