ChemBioChem
ChemBioChem
5 years ago

Chaperone-Polymer-Assisted, Photodriven DNA Strand Displacement

Chaperone-Polymer-Assisted, Photodriven DNA Strand Displacement
Hiroyuki Asanuma, Atsushi Maruyama, Hiromu Kashida, Bohao Cheng, Naohiko Shimada
Photodriven DNA strand displacement by using a 2′,6′-dimethylazobenzene-tethered strand and poly(l-lysine)-graft-dextran (PLL-g-Dex) as a chaperone is reported. Rapid strand displacement was reversibly induced by UV and visible-light irradiation without any toehold portion. To further improve the method, the concentration of PLL-g-Dex and the number of equivalents of the photoresponsive strand were optimised. Optimally, 64 % strand displacement was reversibly induced by alternating UV and visible-light irradiation. Light movements: With the collaboration of DNA containing azobenzene derivatives and chaperone polymer poly(l-lysine)-graft-dextran, high-performance photoregulated DNA strand displacement is achieved without any toehold strand. Rapid, reversible strand displacement is induced by alternating UV and visible-light irradiation.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700202

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