5 years ago

High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation

High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation
Stefan Zauscher, Lei Tang, Luis A. Navarro, Ashutosh Chilkoti
We present terminal deoxynucleotidyl transferase-catalyzed enzymatic polymerization (TcEP) for the template-free synthesis of high-molecular-weight, single-stranded DNA (ssDNA) and demonstrate that it proceeds by a living chain-growth polycondensation mechanism. We show that the molecular weight of the reaction products is nearly monodisperse, and can be manipulated by the feed ratio of nucleotide (monomer) to oligonucleotide (initiator), as typically observed for living polymerization reactions. Understanding the synthesis mechanism and the reaction kinetics enables the rational, template-free synthesis of ssDNA that can be used for a range of biomedical and nanotechnology applications. Biomolecular polymers: Template-free terminal deoxynucleotidyl transferase (TdT) catalyzes the synthesis of high-molecular-weight single-stranded DNA (ssDNA). The reaction parallels the kinetics of synthetic, living polymerizations and makes it possible to manipulate the molecular weight and molecular weight distribution of the ssDNA by the initial concentrations of the initiator, monomers, and enzyme.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201700991

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