5 years ago

Inside Cover: Endo-α-Mannosidase-Catalyzed Transglycosylation (ChemBioChem 14/2017)

Inside Cover: Endo-α-Mannosidase-Catalyzed Transglycosylation (ChemBioChem 14/2017)
Kiichiro Totani, Akira Seko, Yuta Kasahara, Yoichi Takeda, Yukishige Ito, Ichiro Matsuo, Yayoi Yoshimura, Shogo Iwamoto
The inside cover picture shows a transglycosylation reaction catalyzed by Golgi endo-α-mannosidase (G-EM). We have demonstrated that G-EM hydrolysis activity can be employed to mediate transglycosylation by using α-fluorinated glucosyl mannoside as a donor. Systematic screening of various point mutants revealed that the E407D mutant had excellent transglycosylation activity. This optimized mutant is a versatile tool for synthesizing glucosylated high-mannose-type glycans. More information can be found in the communication by I. Matsuo et al. on page 1376 in Issue 14, 2017 (DOI: 10.1002/cbic.201700111).

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700334

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.