Fluorescent sensor array for discrimination of biothiols based on poly(thymine/cytosine)-templated copper nanoparticles
Publication date: Available online 12 November 2018
Source: Analytica Chimica Acta
Author(s): Hongyan Xi, Xin Li, Qingyun Liu, Zhengbo Chen
In medicine, the detection and identification of biothiols have received increasing attention due to their crucial roles in life activities. Hence, we present a facile, rapid, and effective method for identification of five kinds of biothiols including glutathione (GSH), L-cysteine (Cys), dithiothreitol (DTT), 2-mercaptoethanol (MCE), and 3-mercaptopropionic acid (MPA). The proposed sensor array is fabricated based on single-stranded poly (thymine/cytosine)-templated fluorescent copper nanoparticles (CuNPs), which are controlled through thymine-Hg2+-thymine (T-Hg2+-T) and cytosine-Ag+-cytosine (C-Ag+-C) coordination. The results show that single-stranded poly T and poly C are used as a highly efficient template, and ascorbic acid acts as a reductant to form CuNPs. Owing to the different thiol binding affinity between Hg2+ (pKd=3.90) and Ag+ (pKd=5.50), the sensor array produces a unique pattern of fluorescence variations when it interacts with the five biothiols. Linear discriminant analysis (LDA) is applied to analyze the fluorescence pattern and generated a clustering map for a clear identification of the five biothiols. By employing this “turn-on” sensor array, five thiols were successfully discirminated at the 50 nM level in buffer solution and serum samples.
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