Smart wound dressing based on κ–carrageenan/locust bean gum/cranberry extract for monitoring bacterial infections
Publication date: Available online 12 November 2018
Source: Carbohydrate Polymers
Author(s): Karine Modolon Zepon, Maryane Modolon Martins, Morgana Souza Marques, Julia Maia Heckler, Fernando Dal Pont Morisso, Marina Griebeler Moreira, Ana Luiza Ziulkoski, Luiz Alberto Kanis
A smart wound dressing based on carrageenan (κC), locust bean gum (LBG), and cranberry extract (CB) for monitoring bacterial wound infections was developed and characterized using UV-visible spectroscopy, FT-IR, and SEM. The mechanical, swelling, cytotoxic and pH sensor properties were also investigated. UV-visible spectra demonstrated that the obtained κC:LBG:CB hydrogel film exhibited a visible change of colors as it was immersed in PBS solution pH 5.0, 7.3 and 9.0. The spectra of FT-IR suggested that chemical interactions had occurred between κC and CB extract. The obtained κC:LBG:CB hydrogel film exhibited adequate mechanical properties and a swelling behavior dependent on pH. Cytotoxicity tests indicated that κC:LBG:CB hydrogel film had dose-dependent cytotoxicity against NIH 3T3 fibroblast cells. The in vitro studies using Staphylococcus aureus and Pseudomonas aeruginosa demonstrated that the color changes of the κC:LBG:CB hydrogel film could be observed by naked eyes, confirming the potential use of the obtained hydrogel film as a visual system for monitoring bacterial wound infections.