Structural Insights into the Recognition of N2-Aryl- and C8-Aryl DNA Lesions by the Repair Protein XPA/Rad14

5 years ago

Structural Insights into the Recognition of N2-Aryl- and C8-Aryl DNA Lesions by the Repair Protein XPA/Rad14

Thomas Carell, Sabine Schneider, Nina Simon, Charlotte Ebert

Aromatic amines are strongly carcinogenic. They are activated in the liver to give reactive nitrenium ions that react with nucleobases within the DNA duplex. The reaction occurs predominantly at the C8 position of the dG base, thereby giving C8-acetyl-aryl- or C8-aryl-dG adducts in an electrophilic aromatic substitution reaction. Alternatively, reaction with the exocyclic 2-NH2 group is observed. Although the C8 adducts retain base-pairing properties, base pairing is strongly compromised in the case of the N2 adducts. Here we show crystal structures of two DNA lesions, N2-acetylnaphthyl-dG and C8-fluorenyl-dG, within a DNA duplex recognized by the repair protein Rad14. The structures confirm that two molecules of the repair protein recognize the lesion and induce a 72 or 78° kink at the site of the damage. Importantly, the same overall kinked structure is induced by binding of the repair proteins, although the structurally different lesions result in distinct stacking interactions of the lesions within the duplex. The results suggest that the repair protein XPA/Rad14 is a sensor that recognizes flexibility. The protein converts the information that structurally different lesions are present in the duplex into a unifying sharply kinked recognition motif. Crystal structures of nucleotide excision repair factor XPA/Rad14 in complex with C8-aryl and N2-acetyl-aryl-dG DNA gave insights into the interactions. Binding of two Rad14 molecules to the lesion site induces a kink in the DNA and recruits the repair mechanism. XPA/Rad14 thus acts as a duplex flexibility sensor.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/cbic.201700169