Discrimination of VOCs of the Plectranthus grandis by hydrodistillation, HS-SPME and cytotoxic activity
Publication date: January 2019
Source: Industrial Crops and Products, Volume 127
Author(s): André L.M. Camelo, Francisco C. Oliveira, Francisco T. Correia, Francisco G. Barbosa, Jair Mafezoli, Izaura C.N. Diógenes, Ana J. Araújo, Letícia V. Costa-Lotufo, Elisane Longhinotti
In this work, a multivariate data analysis to discriminate 73 volatile organic compounds (VOCs) of roots, stems and leaves of the Plectranthus grandis species which were extracted by hydrodistillation and headspace solid phase microextraction (HS-SPME) methods and identified by gas chromatography (GC) using a flame ionization detector (FID) and mass spectrometry detector (MS). Accordingly, seventeen VOCs (23.3%) were extracted exclusively by HS-SPME, twenty (27.4%) by hydrodistillation and thirty-six (49.3%) of the identified compounds were extracted by both methods While SPME was more suitable for the more volatile compounds, hydrodistillation was better to separate the higher molar mass compounds. The greatest difference in the chemical composition was noticed in the stem samples, as can be ascertained from the hierarchical cluster analysis (HCA) where two distinct groups were observed. A preliminary test of the antitumor potential of the essential oils extracted by hydrodistillation of the roots and stems was performed and just a moderate cytotoxic activity against the human tumor cell lines colon adenocarcinoma (HCT−116), ovarian carcinoma (OVCAR−8) and brain (SF−295) was observed.