3 years ago

Metabolic fingerprinting of carp and rainbow trout seminal plasma

Mariola A. Dietrich, Paulina Samczuk, Michal Ciborowski, Joanna Nynca, Ewa Parfieniuk, Karolina Pietrowska, Tomasz Kowalczyk, Adam Kretowski, Andrzej Ciereszko

Publication date: Available online 12 November 2018

Source: Aquaculture

Author(s): Mariola A. Dietrich, Paulina Samczuk, Michal Ciborowski, Joanna Nynca, Ewa Parfieniuk, Karolina Pietrowska, Tomasz Kowalczyk, Adam Kretowski, Andrzej Ciereszko

Abstract

Rainbow trout and common carp are commercially important aquaculture fish species. To the best of our knowledge, the global composition of fish seminal plasma (SP) metabolites has never been studied. The aim of this study was to identify metabolites in carp and rainbow trout SP and to compare their relative abundances through the use of liquid chromatography-mass spectrometry (LC-MS)-based metabolic fingerprinting. Different solvents and sample/solvent ratios were tested for metabolite extraction. The largest number of metabolic features and the highest signal reproducibility were achieved using a 1:1 sample/acetone ratio for extraction. Accurate masses were searched against metabolite databases, and the identities of 48 and 40 metabolites for rainbow trout and carp SP, respectively, were confirmed using LC-MS/MS or through the standards. Most of the identified metabolites have not been previously described in fish SP. Several metabolites (amino acids, acylcarnitines, vitamins) were found in the SP of both species. However, differences between carp and rainbow trout SP metabolic fingerprints were observed. Certain metabolites appeared to be species-specific, e.g., propionic acid for carp and adipic and picolinic acids, homocitrulline and niacin for rainbow trout. Functional analysis revealed the involvement of SP metabolites in free radical scavenging, lipid and energy metabolism, molecular transport, cell death and survival, and inflammatory response. The identification of a high number of carp and rainbow trout seminal plasma metabolites provides new opportunities to develop novel biomarkers for sperm quality, optimise artificial reproduction and develop efficient cryopreservation procedures for these important aquaculture species.

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