3 years ago

Single versus double experimental bile duct ligation model for inducing bacterial translocation

J.m. Seguí-ripoll, P. Zapater-hernández, A. Candela-gomis, L. Compañ-catalá, R. Francés-guarinos, A. Payá-romá, A. Compañ-rosique, J. Such-ronda

Publication date: Available online 14 November 2018

Source: The American Journal of Surgery

Author(s): J.M. Seguí-Ripoll, P. Zapater-Hernández, A. Candela-Gomis, L. Compañ-Catalá, R. Francés-Guarinos, A. Payá-Romá, A. Compañ-Rosique, J. Such-Ronda


Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation.


Fifty-six male Sprague Dawley rats underwent one of three surgical interventions: a) proximal double ligation and section of the common bile duct; b) proximal simple ligation of the bile duct; and c) sham operation. Bacterial translocation was measured by cultures of mesenteric lymph nodes, blood, spleen and liver. Stool culture and histological analysis of liver damage were also performed.


The incidence of bacterial translocation in SBL and DBDL groups was 23,5% and 25% respectively . Mortality was similar between ligation groups (11.2% versus 10%). Liver cirrhosis developed in the group of double ligation and section (100% of the animals at 4 weeks), while portal hypertension appeared starting at week 3. None of the animals submitted to simple ligation developed liver cirrhosis.


Simple bile duct ligation is associated with a similar incidence of bacterial translocation as double ligation, but without cirrhosis or portal hypertension.

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