3 years ago

Single versus double experimental bile duct ligation model for inducing bacterial translocation

J.m. Seguí-ripoll, P. Zapater-hernández, A. Candela-gomis, L. Compañ-catalá, R. Francés-guarinos, A. Payá-romá, A. Compañ-rosique, J. Such-ronda

Publication date: Available online 14 November 2018

Source: The American Journal of Surgery

Author(s): J.M. Seguí-Ripoll, P. Zapater-Hernández, A. Candela-Gomis, L. Compañ-Catalá, R. Francés-Guarinos, A. Payá-Romá, A. Compañ-Rosique, J. Such-Ronda

Abstract
Background

Double common bile duct ligation plus section in rats is used as a model for bacterial translocation, a phenomenon that has been correlated with the degree of liver damage. This study analyzes whether a simpler variant of the technique is also a valid model to study bacterial translocation.

Methods

Fifty-six male Sprague Dawley rats underwent one of three surgical interventions: a) proximal double ligation and section of the common bile duct; b) proximal simple ligation of the bile duct; and c) sham operation. Bacterial translocation was measured by cultures of mesenteric lymph nodes, blood, spleen and liver. Stool culture and histological analysis of liver damage were also performed.

Results

The incidence of bacterial translocation in SBL and DBDL groups was 23,5% and 25% respectively . Mortality was similar between ligation groups (11.2% versus 10%). Liver cirrhosis developed in the group of double ligation and section (100% of the animals at 4 weeks), while portal hypertension appeared starting at week 3. None of the animals submitted to simple ligation developed liver cirrhosis.

Conclusions

Simple bile duct ligation is associated with a similar incidence of bacterial translocation as double ligation, but without cirrhosis or portal hypertension.

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.