3 years ago

His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples

Eylem Turan

Abstract

In the epitope imprinting method, a short peptide, even without a defined tertiary structure, can be used as the template to produce protein‐selective polymers. In this study, His‐tag‐epitope imprinted magnetic nanoparticles (EMIP‐MNPs) were prepared via epitope imprinting method onto the surface of the magnetic nanoparticles for specific recognition and separation of thyroid peroxidase antigens (TPO). 3‐(Trimethoxysilyl) propyl methacrylate (TMSPM), copper (II) acrylate (Cu(II)Ac) and N‐isopropylacrylamide (NIPAAm) were chosen as functional monomers. Ethyleneglycoldimethacrylate (EGDMA) and 2,2’‐azobisisobutyronitrile (AIBN) were selected as crosslinking agent and initiator, respectively. The EMIP‐MNPs were prepared within 30 min by epitope imprinting method which was shorter than the surface imprinting method. The prepared EMIP‐MNPs were thermosensitive and showed selective affinity to target TPO antigens. Moreover, the EMIP‐MNPs were easily and quickly recognized and separated to target antigens from the whole blood sample.

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/slct.201801557

DOI: 10.1002/slct.201801557

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