Journal of Proteome Research
Journal of Proteome Research
5 years ago

Metabolomics and Lipidomics Profiling of a Combined Mitochondrial Plus Endoplasmic Reticulum Fraction of Human Fibroblasts: A Robust Tool for Clinical Studies

Metabolomics and Lipidomics Profiling of a Combined Mitochondrial Plus Endoplasmic Reticulum Fraction of Human Fibroblasts: A Robust Tool for Clinical Studies
Guy Lenaers, Charlotte Veyrat-Durebex, Stéphanie Chupin, Hélène Blasco, Pascal Reynier, Cinzia Bocca, Gilles Simard, Judith Kouassi Nzoughet
Mitochondria and endoplasmic reticulum (ER) are physically and functionally connected. This close interaction, via mitochondria-associated membranes, is increasingly explored and supports the importance of studying these two organelles as a whole. Metabolomics and lipidomics are powerful approaches for the exploration of metabolic pathways that may be useful to provide deeper information on these organelles’ functions, dysfunctions, and interactions. We developed a quick and simple experimental procedure for the purification of a mitochondria-ER fraction from human fibroblasts. We applied combined metabolomics and lipidomics analyses by mass spectrometry with excellent reproducibility. Seventy-two metabolites and 418 complex lipids were detected with a mean coefficient of variation around 12%, among which many were specific to the mitochondrial metabolism. Thus this strategy based on robust mitochondria-ER extraction and “omics” combination will be useful for investigating the pathophysiology of complex diseases.

Publisher URL: http://dx.doi.org/10.1021/acs.jproteome.7b00637

DOI: 10.1021/acs.jproteome.7b00637

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