3 years ago

Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells

Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells
Maik Herbig, Sandra Tavares, José B. Pereira-Leal, Jochen Guck, André Filipe Vieira, Anna Verena Taubenberger, António Polónia, Florence Janody, Oliver Otto, Margarida Araújo, Joana Paredes, Joana Cardoso, Catarina Brás-Pereira, Nuno Pimpao Martins, Clara Barreto
Studies of the role of actin in tumour progression have highlighted its key contribution in cell softening associated with cell invasion. Here, using a human breast cell line with conditional Src induction, we demonstrate that cells undergo a stiffening state prior to acquiring malignant features. This state is characterized by the transient accumulation of stress fibres and upregulation of Ena/VASP-like (EVL). EVL, in turn, organizes stress fibres leading to transient cell stiffening, ERK-dependent cell proliferation, as well as enhancement of Src activation and progression towards a fully transformed state. Accordingly, EVL accumulates predominantly in premalignant breast lesions and is required for Src-induced epithelial overgrowth in Drosophila. While cell softening allows for cancer cell invasion, our work reveals that stress fibre-mediated cell stiffening could drive tumour growth during premalignant stages. A careful consideration of the mechanical properties of tumour cells could therefore offer new avenues of exploration when designing cancer-targeting therapies.

Publisher URL: http://www.nature.com/articles/ncomms15237

DOI: 10.1038/ncomms15237

You might also like
Never Miss Important Research

Researcher is an app designed by academics, for academics. Create a personalised feed in two minutes.
Choose from over 15,000 academics journals covering ten research areas then let Researcher deliver you papers tailored to your interests each day.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.