3 years ago

Biochemical characterization and comparison of aspartylglucosaminidases secreted in venom of the parasitoid wasps <i>Asobara tabida</i> and <i>Leptopilina heterotoma</i>

Geneviève Prévost, Dominique Colinet, Séverine Lemauf, Quentin Coulette, Jean-Luc Gatti, Marylène Poirié, Caroline Anselme

by Quentin Coulette, Séverine Lemauf, Dominique Colinet, Geneviève Prévost, Caroline Anselme, Marylène Poirié, Jean-Luc Gatti

Aspartylglucosaminidase (AGA) is a low-abundance intracellular enzyme that plays a key role in the last stage of glycoproteins degradation, and whose deficiency leads to human aspartylglucosaminuria, a lysosomal storage disease. Surprisingly, high amounts of AGA-like proteins are secreted in the venom of two phylogenetically distant hymenopteran parasitoid wasp species, Asobara tabida (Braconidae) and Leptopilina heterotoma (Cynipidae). These venom AGAs have a similar domain organization as mammalian AGAs. They share with them key residues for autocatalysis and activity, and the mature α- and β-subunits also form an (αβ)2 structure in solution. Interestingly, only one of these AGAs subunits (α for AtAGA and β for LhAGA) is glycosylated instead of the two subunits for lysosomal human AGA (hAGA), and these glycosylations are partially resistant to PGNase F treatment. The two venom AGAs are secreted as fully activated enzymes, they have a similar aspartylglucosaminidase activity and are both also efficient asparaginases. Once AGAs are injected into the larvae of the Drosophila melanogaster host, the asparaginase activity may play a role in modulating their physiology. Altogether, our data provide new elements for a better understanding of the secretion and the role of venom AGAs as virulence factors in the parasitoid wasps’ success.

Publisher URL: http://journals.plos.org/plosone/article

DOI: 10.1371/journal.pone.0181940

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