In-Phase Ultra High-Resolution In Vivo NMR

Angewandte Chemie - International Edition

5 years ago

In-Phase Ultra High-Resolution In Vivo NMR

Ioana Fugariu, Daniel Lane, Wolfgang Bermel, Ronald Soong, Andre J. Simpson

Although current NMR techniques allow organisms to be studied in vivo, magnetic susceptibility distortions, which arise from inhomogeneous distributions of chemical moieties, prevent the acquisition of high-resolution NMR spectra. Intermolecular single quantum coherence (iSQC) is a technique that breaks the sample's spatial isotropy to form long range dipolar couplings, which can be exploited to extract chemical shift information free of perturbations. While this approach holds vast potential, present practical limitations include radiation damping, relaxation losses, and non-phase sensitive data. Herein, these drawbacks are addressed, and a new technique termed in-phase iSQC (IP-iSQC) is introduced. When applied to a living system, high-resolution NMR spectra, nearly identical to a buffer extract, are obtained. The ability to look inside an organism and extract a high-resolution metabolic profile is profound and should find applications in fields in which metabolism or in vivo processes are of interest. Live-organism metabolite elucidation: Magnetic susceptibility distortions stemming from an organism's complex nature prevent the acquisition of high-resolution in vivo NMR spectra. A new technique, intermolecular single quantum coherence (IP-iSQC), yields in vivo spectra nearly identical to analogous buffer extracts. The approach provides a high-resolution molecular window into real-time in vivo processes and metabolism.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201701097