4 years ago

Bioluminescent Antibodies for Point-of-Care Diagnostics

Bioluminescent Antibodies for Point-of-Care Diagnostics
Rudolf Griss, Kai Johnsson, Qiuliyang Yu, Alberto Schena, Lin Xue
We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the latter being labeled with a synthetic fluorescent competitor of the antigen. Binding of the antigen, here synthetic drugs, by the sensor displaces the tethered fluorescent competitor from the antibody and disrupts bioluminescent resonance energy transfer (BRET) between the luciferase and fluorophore. The semisynthetic sensors display a tunable response range (submicromolar to submillimolar) and large dynamic range (ΔRmax>500 %), and they permit the quantification of analytes through spotting of the samples onto paper followed by analysis with a digital camera. Paper test: Ratiometric bioluminescent sensors with high selectivity, tunable response range, and a large dynamic range were developed based on antibodies. Moreover, when incorporated into paper-based point-of-care devices, the antibody-based sensors permit the rapid, reliable, and portable quantification of analytes in serum and blood samples using a digital camera.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201702403

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.