3 years ago

Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection

Sectioning of cultured cell monolayer using photo-acid-generating substrate and micro-patterned light projection
A novel cell processing tool to cut and section a cultured cell monolayer was implemented by the micro-projection of grid pattern onto a substrate functionalized with photo-acid-generating polymer. In adequate conditions of irradiation dosage and line width of grid pattern, Madin-Darby canine kidney cell monolayer was cut into cell clumps with similar size corresponding to the projected grid pattern. The sectioned monolayers were collected as cell clumps with the similar size from the substrate maintaining their viability enough to outgrow after subculture to the other dish. Also the influence of cutout size on the growth rate of collected cell clumps after subculture was also investigated. Further, passage of human induced pluripotent stem cells with size uniformity of the colonies was achieved by using this sectioning method without critical influence on their viability and undifferentiated state.

Publisher URL: www.sciencedirect.com/science

DOI: S0014305716311478

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