4 years ago

Penetration and toxicity of chitosan and its derivatives

Penetration and toxicity of chitosan and its derivatives
Chitosan (CH) is a highly attractive biopolymer with multiple reactive groups which can be used to develop targeted nanoparticles. Positively and negatively charged chitosan derivatives can be used to obtain nanoparticles with different properties. The aim of this work was to determine the role of charge, hydrophobicity and solubility at neutral pH in CH cell penetration and cytotoxicity. We showed that positively charged unmodified CH does not penetrate the cells and is not toxic. Quaternization of CH (CHq) led to the increase in solubility, cell penetration, and cytotoxicity manifested by reactive oxygen species production, cell cycle arrest and inhibition of cell proliferation. Modification of CHq with hydrophobic residues significantly diminished cytotoxic activity however preserved cell penetration. Negatively charged CH derivative succinyl chitosan (CHs) penetrated the cells highly efficiently and demonstrated the stimulation of cell proliferation. Thus, quaternized hydrophobic CH derivatives can be recommended to develop positively charged delivery systems, while any negatively charged chitosans are safe to design nanoparticles.

Publisher URL: www.sciencedirect.com/science

DOI: S0014305716312423

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.