Angewandte Chemie - International Edition
Angewandte Chemie - International Edition
5 years ago

Specific Identification of Glycoproteins Bearing the Tn Antigen in Human Cells

Specific Identification of Glycoproteins Bearing the Tn Antigen in Human Cells
Jiangnan Zheng, Ronghu Wu, Haopeng Xiao
Glycoproteins contain a wealth of valuable information regarding the development and disease status of cells. In cancer cells, some glycans (such as the Tn antigen) are highly up-regulated, but this remains largely unknown for glycoproteins with a particular glycan. Herein, an innovative method combining enzymatic and chemical reactions was first designed to enrich glycoproteins with the Tn antigen. Using synthetic glycopeptides with O-GalNAc (the Tn antigen) or O-GlcNAc, we demonstrated that the method is selective for glycopeptides with O-GalNAc and can distinguish between these two modifications. The diagnostic ions from the tagged O-GalNAc further confirmed the effectiveness of the method and confidence in the identification of glycopeptides with the Tn antigen by mass spectrometry. Using this method, we identified 96 glycoproteins with the Tn antigen in Jurkat cells. The method can be extensively applied in biological and biomedical research. Both sides of the story: Glycoproteins contain a wealth of valuable information regarding the disease status of cells. The Tn antigen is highly expressed in cancer cells, but glycoproteins bearing this important antigen remain unexplored. Furthermore, the Tn antigen is indistinguishable from O-GlcNAc by MS. A novel method integrating enzymatic and chemical reactions for specific identification of glycoproteins with the Tn antigen by LC-MS/MS was developed.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/anie.201702191

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