3 years ago

Alleviation of salt stress and changes in glycyrrhizin accumulation by arbuscular mycorrhiza in liquorice (Glycyrrhiza glabra) grown under salinity stress

Setareh Amanifar, Maryam Khodabandeloo, Ehsan Mohsenifard, Mohammad Sadegh Askari, Mohsen Ashrafi

Publication date: Available online 8 January 2019

Source: Environmental and Experimental Botany

Author(s): Setareh Amanifar, Maryam Khodabandeloo, Ehsan Mohsenifard, Mohammad Sadegh Askari, Mohsen Ashrafi


The symbiotically interaction of arbuscular mycorrhiza (AM) with liquorice (Glycyrrhiza glabra) which is widely used in traditional Iranian medicine, may have a good potential to enhance host plant growth and resistance to salt stress and may regulate the synthesis and accumulation pattern of secondary metabolites in plant tissues. The aims of this study were to assess the effect of AM fungus Funneliforms mosseae on liquorice plant's growth and nutrition, and the expression of important genes participated in the glycyrrhizin biosynthesis under salinity stress. The salinity stress induced a decreased trend in root fungal colonization, growth parameters, K and P concentration and K+/Na+ ratios. Moreover, salinity caused a significant increase in electrolyte leakage and Na+ concentration in shoot and root of liquorice. AM colonization considerably increased P and K concentrations under salinity stress and promoted shoot proline accumulation and a higher K+/Na+ ratio and concentration of glycyrrhizin. The expression of three genes including beta-amyrin synthas (bAS), squalene synthase1 (SQS1) and P450 under different treatments were assessed by qRT-PCR. The highest increase in bAS and P450 gene expression was observed in stressed mycorrhizal plants. The expression of SQS1 was higher in stress condition compared to non-stressed plants. This study indicated the ameliorating effects of arbuscular mycorrhizal symbiosis in liquorice plant under salinity stress. The symbiosis of liquorice with AM fungi can be suggested as an alternative to enhance liquorice quality for pharmaceutical purposes and a practical approach for exploiting saline soils.

Graphical abstract

Graphical abstract for this article

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.