Extending the osmometer method for assessing drought tolerance in herbaceous species

Abstract

Community-scale surveys of plant drought tolerance are essential for understanding semi-arid ecosystems and community responses to climate change. Thus, there is a need for an accurate and rapid methodology for assessing drought tolerance strategies across plant functional types. The osmometer method for predicting leaf osmotic potential at full turgor (π_o), a key metric of leaf-level drought tolerance, has resulted in a 50-fold increase in the measurement speed of this trait; however, the applicability of this method has only been tested in woody species and crops. Here, we assess the osmometer method for use in herbaceous grassland species and test whether π_o is an appropriate plant trait for understanding drought strategies of herbaceous species as well as species distributions along climate gradients. Our model for predicting leaf turgor loss point (π_TLP) from π_o (π_TLP = 0.80π_o–0.845) is nearly identical to the model previously presented for woody species. Additionally, π_o was highly correlated with π_TLP for graminoid species (π_tlp = 0.944π_o–0.611; r² = 0.96), a plant functional group previously flagged for having the potential to cause erroneous measurements when using an osmometer. We report that π_o, measured with an osmometer, is well correlated with other traits linked to drought tolerance (namely, leaf dry matter content and leaf vulnerability to hydraulic failure) as well as climate extremes linked to water availability. The validation of the osmometer method in an herb-dominated ecosystem paves the way for rapid community-scale surveys of drought tolerance across plant functional groups, which could improve trait-based predictions of ecosystem responses to climate change.