3 years ago

Identification of new bioactive peptides from Kefir milk through proteopeptidomics: bioprospection of antihypertensive molecules

Fernanda Gobbi Amorim, Luciana Barbosa Coitinho, Ananda Tissianel Dias, Andreia Gomes Freitas Friques, Brenna Lepaus Monteiro, Lucas Cunha Dias De Rezende, Thiago De Melo Costa Pereira, Bianca Prandi Campagnaro, Edwin De Pauw, Elisardo Corral Vasquez, Loïc Quinton

Publication date: Available online 8 January 2019

Source: Food Chemistry

Author(s): Fernanda Gobbi Amorim, Luciana Barbosa Coitinho, Ananda Tissianel Dias, Andreia Gomes Freitas Friques, Brenna Lepaus Monteiro, Lucas Cunha Dias de Rezende, Thiago de Melo Costa Pereira, Bianca Prandi Campagnaro, Edwin De Pauw, Elisardo Corral Vasquez, Loïc Quinton

Abstract

Kefir, a probiotic beverage prepared from fermented milk, has been associated with antihypertensive activity. However, the bioactive molecules responsible for this activity still remain unclear. Therefore, in this study we aim to evaluate the mechanisms of the antihypertensive effects of Kefir in the two-kidney one-clip hypertension model, and to bioprospect for bioactive peptides identified by proteomic methodologies. Treatment with Kefir was performed via gavage, and resulted in a 37 mmHg reduction in systolic arterial pressure and 19% inhibition of angiotensin converting enzyme (ACE) activity. For the proteopeptidomic study, the protein extract of Kefir beverage and non-fermented bovine milk were analysed by MALDI-TOF mass spectrometry, and their tryptic digestion products sequenced via Shotgun proteomics (Q-Exactive mass spectrometer). A list of 35 peptides with potential hypertensive activity due to ACE inhibition were identified. These results demonstrate the benefits of Kefir products, and may guide the design of new antihypertensive drugs.

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