Transcriptome-wide identification of differentially expressed genes in Procambarus clarkii in response to chromium challenge
Publication date: April 2019
Source: Fish & Shellfish Immunology, Volume 87
Author(s): Xun Meng, Liang Hong, Ting-Ting Yang, Yu Liu, Ting Jiao, Xiao-Hua Chu, Dai-Zhen Zhang, Jia-Lian Wang, Bo-Ping Tang, Qiu-Ning Liu, Wei-Wei Zhang, Wen-Fei He
Because of the high protein content and rich meat quality of crayfish Procambarus clarkii, it has become widely popular in China in recent years and has a high economic value. When P. clarkii is stimulated by heavy metals, it reacts to oxidation. P. clarkii has evolved antioxidant defense systems, including antioxidant enzymes such as catalase (CAT). The hexavalent form of Cr (VI) is a pathogenic factor that is of particular concern in aqueous systems because of its great toxicity to living organisms. In this study, we characterized the transcriptome of P. clarkii using a RNA sequencing method and performed a comparison between K2Cr2O7-treated samples and controls. In total, 34,237 unigenes were annotated. We identified 5098 significantly differentially expressed genes (DEGs), including 2536 and 2562 were significantly up-regulated and down-regulated, respectively. In addition, quantitative real time-PCR (qRT-PCR) confirmed the up-regulation of a random selection of DEGs. Our results contribute to a more comprehensive understanding of the antioxidant defense system used by P. clarkii in response to heavy metal stress.
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