3 years ago

Heterotrophic bacterial production measured on soil microaggregates sampled using a Biological Laser Printer

Michael T. Montgomery, Preston A. Fulmer, Joel D. Gaston, Russell K. Pirlo, Bradley R. Ringeisen

Publication date: Available online 8 January 2019

Source: Soil Biology and Biochemistry

Author(s): Michael T. Montgomery, Preston A. Fulmer, Joel D. Gaston, Russell K. Pirlo, Bradley R. Ringeisen

Abstract

Although routinely measured in aquatic systems over the last 30 years, heterotrophic bacterial production (3H-leucine method) has only more recently been measured in terrestrial ecosystems to elucidate the role of soil biogeochemical processes in global carbon cycles and climate change. Studying bacterial metabolism at the microaggregate scale (100–300 μm) may unmask important microscale biogeochemical relationships amongst bacterial processes and the surrounding soil. Biological laser printer technology was used to sample soil core sections (ca. one mm thick) into ca. 100–300 μm microaggregates. Bacterial production was measured on collections of five microaggregates each (180 samples over 5 cm depth profile) by suspending microaggregates into filtered rainwater with 3H-leucine and incubating for 4 h. Bacterial production varied by over two orders of magnitude amongst groups of five microaggregates collected over the same millimeter scale core slice. Range for all measurements along the core was below detection (0.005) to 897 fg C aggregate d−1. As expected, variability amongst adjacent (within 2 mm of each other) microaggregates groups generally decreased with depth along with the total magnitude of bacterial production rate. Variation in bacterial production at the microaggregate scale was greater than that seen at larger scale (ca. 50 mg; theoretical 3200 microaggregates) sampling of the same core. This is the first demonstration of using a biological laser printer for fine scale sampling of soil core slices and subsequent measurement of bacterial metabolism.

Graphical abstract

Image 1

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.