3 years ago

Fluorometric determination of pesticides and organophosphates using nanoceria as a phosphatase mimic and an inner filter effect on carbon nanodots

Jinchao Wei, Yu Yang, Jiayi Dong, Shuangpeng Wang, Peng Li

Abstract

Nanoceria with a remarkable phosphatase mimicking activity was synthesized and used to catalyze the hydrolysis of phosphate esters in pH 10 solution. The catalytic effect of nanoceria was firstly investigated by selecting p-nitrophenyl phosphate as a model substrate. The pH value, incubation temperature, reaction time, and concentration of nanoceria were optimized. The catalytic effect was then confirmed by using methyl-paraoxon as a substrate. The p-nitrophenol anion released by the enzyme mimic is yellow and exerts an inner filter effect on the fluorescence of the carbon dots (with excitation/emission maxima at 400/520 nm). Response to methyl-paraoxon is linear in the 1.125–26.25 μmol L−1 concentration range. The method was applied to the determination of pesticides in spiked Panax quinquefolius and water samples. Recoveries ranged from 85 to 103% (n = 3). The technique is rapid, reliable, and can be used for on-site detection of pesticides and organophosphates.

Graphical abstract

Schematic presentation of a fluorometric technique for the detection of organophosphate compound and pesticide using nanoceria as a phosphatase mimic and an inner filter effect on the blue fluorescence of carbon dots (with excitation/emission maxima at 400/520 nm).

Publisher URL: https://link.springer.com/article/10.1007/s00604-018-3175-x

DOI: 10.1007/s00604-018-3175-x

You might also like
Discover & Discuss Important Research

Keeping up-to-date with research can feel impossible, with papers being published faster than you'll ever be able to read them. That's where Researcher comes in: we're simplifying discovery and making important discussions happen. With over 19,000 sources, including peer-reviewed journals, preprints, blogs, universities, podcasts and Live events across 10 research areas, you'll never miss what's important to you. It's like social media, but better. Oh, and we should mention - it's free.

  • Download from Google Play
  • Download from App Store
  • Download from AppInChina

Researcher displays publicly available abstracts and doesn’t host any full article content. If the content is open access, we will direct clicks from the abstracts to the publisher website and display the PDF copy on our platform. Clicks to view the full text will be directed to the publisher website, where only users with subscriptions or access through their institution are able to view the full article.