Chemistry - A European Journal
Chemistry - A European Journal
5 years ago

pH-Responsive Fluorescence Enhancement in Graphene Oxide–Naphthalimide Nanoconjugates: A Fluorescence Turn-On Sensor for Acetylcholine

pH-Responsive Fluorescence Enhancement in Graphene Oxide–Naphthalimide Nanoconjugates: A Fluorescence Turn-On Sensor for Acetylcholine
Silja Abraham, Sreejith Mangalath, Joshy Joseph
A pH-sensitive, fluorescence “turn-on” sensor based on a graphene oxide–naphthalimide (GO–NI) nanoconjugate for the detection of acetylcholine (ACh) by monitoring the enzymatic activity of acetylcholinesterase (AChE) in aqueous solution is reported. These nanoconjugates were synthesized by covalently anchoring picolyl-substituted NI derivatives on the GO/reduced GO surface through a 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling strategy, and the morphological and photophysical properties were studied in detail. Synergistic effects of π–π interactions between GO and the NI chromophore, and efficient photoinduced electron- and energy-transfer processes, were responsible for the strong quenching of fluorescence of these nanoconjugates, which were perturbed under acidic pH conditions, leading to significant enhancement of fluorescence emission. This nanoconjugate was successfully employed for the efficient sensing of pH changes caused by the enzymatic activity of AChE, thereby demonstrating its utility as a fluorescence turn-on sensor for ACh in the neurophysiological range. Activating surfaces: A pH-sensitive, fluorescence “turn-on” sensor based on a graphene oxide–naphthalimide nanoconjugate for the detection of acetylcholine (ACh) by monitoring the enzymatic activity of acetylcholinesterase (AChE) in aqueous solution is reported (see figure). Synergistic effects of π–π interactions and efficient photoinduced electron- and energy-transfer processes are responsible for the strong quenching of fluorescence of these nanoconjugates.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201702198

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