5 years ago

Aminopyrazine Pathway to the Moco Metabolite Dephospho Form A

Aminopyrazine Pathway to the Moco Metabolite Dephospho Form A
Anne Klewe, Thomas Lindel, Tobias Kruse
An efficient synthesis of the molybdopterin/molybdenum cofactor (Moco) oxidation product dephospho Form A is described that assembles the pteridinone system starting from an iodinated aminopyrazine. The sodium salt of dephospho Form A could be purified by precipitation from methanol, which paved the way to the title compound in the 100 mg range. By HPLC, the synthetic material was compared with a sample isolated from a recombinant Moco containing protein. Analysis of dephospho Form A is the only method that allows the quantification of the Moco content of crude cell extracts and recombinant protein preparations. Nine million bicycles of pterin: Quantification of the molybdenum cofactor (Moco) in crude cell extracts and recombinant protein preparations requires a synthetic standard of dephospho Form A, which was synthesized on a new route via an iodinated aminopyrazine. The alkynyl moiety had to be introduced prior to the assembly of the pterin bicycle.

Publisher URL: http://onlinelibrary.wiley.com/resolve/doi

DOI: 10.1002/chem.201702274

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