Gold Nanoparticle Loaded Split-DNAzyme Probe for Amplified miRNA Detection in Living Cells

4 years ago

Gold Nanoparticle Loaded Split-DNAzyme Probe for Amplified miRNA Detection in Living Cells

Changbei Ma, Yanan Wu, Nuli Xie, Yanjing Yang, Jin Huang, Jing Li, Kemin Wang, Ke Quan, Xiaohai Yang

A new class of intracellular nanoprobe, termed AuNP loaded split-DNAzyme probe, was developed to sense miRNA in living cells. Briefly, it consists of an AuNP and substrates hybridized with two half of split DNAzymes. In the absence of target miRNA, the split DNAzymes form an inactive DNAzyme motif with their substrate through partial paring at the end of each strand, and the fluorescence is quenched. Inside the cells, the target miRNA binds with both of the two half of split DNAzymes, forming the active secondary structure in the catalytic cores, which can cleave the substrates, resulting in the rupture of the substrate and recovery of the fluorescence. Meanwhile, the target is released and binds to another inactive DNAzyme motif to drive another cycle of activation. During the cyclic process, a very small number of target miRNAs can initiate the cleavage of many fluorophore-labeled substrate strands from AuNP surface, providing an amplified fluorescent signal of the target miRNA and, thus, offering high detection sensitivity.

Publisher URL: http://dx.doi.org/10.1021/acs.analchem.7b01632

DOI: 10.1021/acs.analchem.7b01632