4 years ago

Functional Detection of TNF Receptor Family Members by Affinity-Labeled Ligands

Peng Liang, Anliang Huang, Hong Zhou, Xinyu Liu, Xiaojun Liu, Lingmo Chang, Yang Xu, Joshua G. Liang
Aberrant expression of TNF family of cytokines has been linked to human diseases, and biologics targeting their signaling have become the best selling drugs globally. However, functional detection with labeled ligands for accurate detection of TNFR family of receptor-expressing target tissues or cell types remains to be developed. Here we show that TNF receptor family members are heat-stable and can be recognized both in vitro and in vivo by their ligands labeled with alkaline phosphatase. Such an approach may be used in lieu of antibodies for the identification of the cell types involved in receptor signaling during disease onset and progression.

Publisher URL: https://www.nature.com/articles/s41598-017-06343-4

DOI: 10.1038/s41598-017-06343-4

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