Nature Communications
Nature Communications
5 years ago

The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks

The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks
Lindsay N. Redman, Lee A. Uranga, Emigdio D. Reyes, Praveen L. Patidar, Shelley L. Lusetti
RecN is a cohesin-like protein involved in DNA double-strand break repair in bacteria. The RecA recombinase functions to mediate repair via homologous DNA strand invasion to form D-loops. Here we provide evidence that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DNA repair. The intermolecular DNA tethering activity of RecN protein described previously cannot fully explain this novel activity since stimulation of RecA function is species-specific and requires RecN ATP hydrolysis. Further, DNA-bound RecA protein increases the rate of ATP hydrolysis catalysed by RecN during the DNA pairing reaction. DNA-dependent RecN ATPase kinetics are affected by RecA protein in a manner suggesting a specific order of protein–DNA assembly, with RecN acting after RecA binds DNA. We present a model for RecN function that includes presynaptic stimulation of the bacterial repair pathway perhaps by contributing to the RecA homology search before ternary complex formation.

Publisher URL: http://www.nature.com/articles/ncomms15282

DOI: 10.1038/ncomms15282

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